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Cytokeratin 18 antibody

Catalog Number:OM249155
OM249155
Discount Price
OM249155-100ul
1~2 Week
100ul
OM249155-50ul
50ul
1~2 Week
Product Profile
Product Name Cytokeratin 18 antibody
Antibody Type Primary Antibodies
Product description Mouse Monoclonal antibody [1E1] to Cytokeratin 18Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Immunogen Recombinant fragment expressed in E.coli corresponding to amino acids 69-372 of human CK18
Key Feature
Clonality Monoclonal
Isotype IgG1
Clone Number 1E1
Host Species Mouse
Tested Applications ICC/IFIHCIHC-PWB
IHC 1:50 :
Species Reactivity Human
Concentration 1mg/ml
Purification Affinity purified
Target Information
Gene Synonyms K18
CYK18
KRT18
Target Cytokeratin 18
Tissue Specificity is specific for amino acids 69-372 of human CK18
Application

Application

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT18 monoclonal antibody.

Application

Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-KRT18 mouse monoclonal antibody.

Application

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-KRT18 mouse monoclonal antibody.

Application

Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-KRT18 mouse monoclonal antibody.

Application

CK18 antibody ( 1E1) at 1:1000 dilution with HepG2 cell lysate

Application

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT18 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT18.

Application

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-KRT18 mouse monoclonal antibody.

Application

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-KRT18 mouse monoclonal antibody.

Application

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-KRT18 mouse monoclonal antibody.

Application

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-KRT18 mouse monoclonal antibody.

Application

Anti-KRT18 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with KRT18

Application

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-KRT18 mouse monoclonal antibody.

Application

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-KRT18 mouse monoclonal antibody.

Application

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KRT18 mouse monoclonal antibody.
Application Notes IHC 1:50 :
Additional Information
Form Liquid
Storage Instructions For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles.
Storage Buffer PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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