Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT18 monoclonal antibody.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT18 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT18.Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-KRT18 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KRT18 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-KRT18 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-KRT18 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-KRT18 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-KRT18 mouse monoclonal antibody.| Product Name | Cytokeratin 18 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [5E8] to Cytokeratin 18Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. |
| Immunogen | Recombinant fragment expressed in E.coli corresponding to amino acids 69-372 of human CK18 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 5E8 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIHC-PWB |
| IHC 1:50 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | K18 CYK18 KRT18 |
|---|---|
| Target | Cytokeratin 18 |
| Tissue Specificity | is specific for amino acids 69-372 of human CK18 |
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT18 monoclonal antibody.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT18 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT18.
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-KRT18 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KRT18 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-KRT18 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-KRT18 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-KRT18 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-KRT18 mouse monoclonal antibody.| Application Notes | IHC 1:50 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
