| Product Name | Cytokeratin 18 antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [CY-90] to Cytokeratin 18, predilutedCytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie. mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Cytokeratin 18 is an acidic keratin which is found primarily in non-squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18. |
| Immunogen | Epidermal carcinoma (A431) and MCF-7 human breast cancer cells (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | CY90 |
| Host Species | Mouse |
| Tested Applications | IHC-FIHC-P |
| IHC-P: This antibody is prediluted and ready to use. An incubation period of 30 minutes at room temperature is recommended. Proteolytic digestion may be required in some paraffin embedded tissue sections. IHC-Fr: This antibody is prediluted and ready to use. An incubation period of 30 minutes at room temperature is recommended. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KRT18 K18 CYK18 Cytokeratin18 P05783 3875 Cytokeratin 18 148070 |
|---|---|
| Target | Cytokeratin 18 |
| Tissue Specificity | In normal tissues, reacts with a large number of epithelial cell types, including ductal and glandular epithelia. This antibody does not react with squamous epithelia. Most of the adenocarcinomas, basal cell carcinomas and benign and malignant epithelial lesions stain with this antibody. |
| Cellular Localization | Cytoplasmic |
| Positive Control | Skin |
|---|---|
| Application Notes | IHC-P: This antibody is prediluted and ready to use. An incubation period of 30 minutes at room temperature is recommended. Proteolytic digestion may be required in some paraffin embedded tissue sections. IHC-Fr: This antibody is prediluted and ready to use. An incubation period of 30 minutes at room temperature is recommended. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Preservative: Sodium Azide. Constituents: Ascites |
