Application
Immunohistochemistry staining of human colon (paraffin sections) using anti-cytokeratin 18Application
Western Blot analysis (reducing conditions) of whole cell lysate using anti-human Cytokeratin 18 Lane 1: HeLa human cervix carcinoma cell line Lane 2: A-431 human epidermoid carcinoma cell line Lane 3: MCF-7 human breast adenocarcinoma cell line| Product Name | Cytokeratin 18 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [DA-7] to Cytokeratin 18Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie. mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Cytokeratin 18 is an acidic keratin which is found primarily in non-squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18. |
| Immunogen | Human breast carcinoma cell line PMC-42 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | DA-7 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHC-PIPWB |
| For ELISA: Use at an assay dependant dilution. For ICC: Use at an assay dependant dilution. For IHC-P: Use at a dilution of 1:10-1:100 For IP: Use at an assay dependant dilution. For WB: Use at a concentration of 1-2µg/ml. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | KRT18 K18 CYK18 Cytokeratin18 P05783 3875 Cytokeratin 18 148070 |
|---|---|
| Target | Cytokeratin 18 |
| Tissue Specificity | Cytokeratin 18 |
Application
Immunohistochemistry staining of human colon (paraffin sections) using anti-cytokeratin 18
Application
Western Blot analysis (reducing conditions) of whole cell lysate using anti-human Cytokeratin 18 Lane 1: HeLa human cervix carcinoma cell line Lane 2: A-431 human epidermoid carcinoma cell line Lane 3: MCF-7 human breast adenocarcinoma cell line| Positive Control | Hela, MCF-7, epithelial tissue |
|---|---|
| Application Notes | For ELISA: Use at an assay dependant dilution. For ICC: Use at an assay dependant dilution. For IHC-P: Use at a dilution of 1:10-1:100 For IP: Use at an assay dependant dilution. For WB: Use at a concentration of 1-2µg/ml. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 containing 15 mM sodium azide |
