Application
Human Skin stained with anti-Keratin 19 antibodyApplication
Human skin stained with anti-cytokeratin 19 antibody, by Immunohistochemistry (FFPE-sections).| Product Name | Cytokeratin 19 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Cytokeratin 19This protein is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis. |
| Immunogen | Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) C terminal (Human). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-P |
| IF: Use at an assay dependent dilution. IHC-P: Use at a dilution of 1/100 for 10 min at RT. Staining of formalin-fixed tissues requires digesting tissue sections with pepsin for 10 min at 37C. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 148020, Cytokeratin 19, KRT19, K19, P08727, CK19, K1CS, MGC15366, 3880, Cytokeratin19 |
|---|---|
| Target | Cytokeratin 19 |
| Cellular Localization | Cytoplasmic |
Application
Human Skin stained with anti-Keratin 19 antibody
Application
Human skin stained with anti-cytokeratin 19 antibody, by Immunohistochemistry (FFPE-sections).| Positive Control | Skin. |
|---|---|
| Application Notes | IF: Use at an assay dependent dilution. IHC-P: Use at a dilution of 1/100 for 10 min at RT. Staining of formalin-fixed tissues requires digesting tissue sections with pepsin for 10 min at 37C. Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4ºC |
| Storage Buffer | Preservative: Sodium Azide; Constituents: 1% BSA, 10mM PBS. pH 7.4 |
