Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT19 monoclonal antibody.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT19 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT19.Application
Anti-KRT19 mouse monoclonal antibody immunofluorescent staining of HELA cells transiently transfected with KRT19Application
Immunohistochemical staining of paraffin-embedded Human bladder tissue using anti-KRT19 mouse monoclonal antibody.| Product Name | Cytokeratin 19 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [2F8] to Cytokeratin 19Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. |
| Immunogen | Recombinant fragment expressed in E.coli corresponding to amino acids 240-390 of human CK19 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2F8 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| ICC/IF (1:100): |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | KRT19 K19 MGC15366 K1CS CK19 |
|---|---|
| Target | Cytokeratin 19 |
| Tissue Specificity | is specific for amino acids 240-390 of human CK19 |
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT19 monoclonal antibody.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT19 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT19.
Application
Anti-KRT19 mouse monoclonal antibody immunofluorescent staining of HELA cells transiently transfected with KRT19
Application
Immunohistochemical staining of paraffin-embedded Human bladder tissue using anti-KRT19 mouse monoclonal antibody.| Application Notes | ICC/IF (1:100): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
