Application
IHC Image using - Detection of Cytokeratin 19 by IHC in human skin| Product Name | Cytokeratin 19 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Mouse Antibody to Human Cytokeratin 19This antibody is specific to a 40 kD protein, which is identified cytokeratin 19. Its epitope maps between amino acids 312-335. This antibody reacts with MCF-7 cells, which are known to contain cytokeratin 19. |
| Immunogen | BALB/C mice were injected with human breast cancer MCF-7 cells. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | A53-B/A2.26 |
| Host Species | Mouse |
| Tested Applications | IHC-P |
| This antibody may be diluted to a titer of 1:25-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Staining of Formalin fixed paraffin embedded tissue sections is enhanced by high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 148020 Cytokeratin 19 KRT19 K19 P08727 CK19 K1CS MGC15366 3880 Cytokeratin19 |
|---|---|
| Target | Cytokeratin 19 |
| Cellular Localization | Cytoplasmic |
| Light Chain Type | kappa |
Application
IHC Image using - Detection of Cytokeratin 19 by IHC in human skin| Positive Control | Skin |
|---|---|
| Application Notes | This antibody may be diluted to a titer of 1:25-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Staining of Formalin fixed paraffin embedded tissue sections is enhanced by high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
