Application
Western Blot analysis (reducing conditions) of whole cell lysate of MCF-7 human breast adenocarcinoma cell line. Lane 1,2: immunostaining with anti-human Cytokeratin 19 (BA-17) Lane 3,4: immunostaining with anti-human Cytokeratin 19 (A53-B/A2)| Product Name | Cytokeratin 19 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [A53 - B / A2] to Cytokeratin 19This protein is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis. |
| Immunogen | Human mammary carcinoma cell line MCF-7. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | A53-B/A2 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHC-PIPWB |
| For ELISA: Use at an assay dependent dilution. For ICC: Use at an assay dependent dilution. For IHC-P: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | 148020 Cytokeratin 19 KRT19 K19 P08727 CK19 K1CS MGC15366 3880 Cytokeratin19 |
|---|---|
| Target | Cytokeratin 19 |
| Cellular Localization | Cytoplasmic |
Application
Western Blot analysis (reducing conditions) of whole cell lysate of MCF-7 human breast adenocarcinoma cell line. Lane 1,2: immunostaining with anti-human Cytokeratin 19 (BA-17) Lane 3,4: immunostaining with anti-human Cytokeratin 19 (A53-B/A2)| Application Notes | For ELISA: Use at an assay dependent dilution. For ICC: Use at an assay dependent dilution. For IHC-P: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline, pH 7.4, containing 15mM sodium azide |
