Application
Detection of cytokeratin 19 in MCF-7 cell lysate by mouse monoclonal antibody BA-17 .| Product Name | Cytokeratin 19 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [BA-17] to Cytokeratin 19This protein is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis. |
| Immunogen | Mammary organoids |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | BA-17 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHC-PIPWB |
| For FACS: Use at an assay dependent dilution. For ICC: Use at an assay dependant dilution. For IHC-P: Use at an assay dependant dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. For IP: Use at an assay dependant dilution. For WB: Use at an assay dependant dilution. Optimal dilutions/concentrations should be determined by the researcher.: |
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| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 148020 Cytokeratin 19 KRT19 K19 P08727 CK19 K1CS MGC15366 3880 Cytokeratin19 |
|---|---|
| Target | Cytokeratin 19 |
| Tissue Specificity | Cytokeratin 19. Not expressed in hepatocytes. |
| Cellular Localization | Cytoplasmic |
Application
Detection of cytokeratin 19 in MCF-7 cell lysate by mouse monoclonal antibody BA-17 .| Application Notes | For FACS: Use at an assay dependent dilution. For ICC: Use at an assay dependant dilution. For IHC-P: Use at an assay dependant dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. For IP: Use at an assay dependant dilution. For WB: Use at an assay dependant dilution. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 containing 15mM Sodium Azide |
