Application
Immunohistochemical analysis of paraffin-embedded H661 xenograft, using KRT2 antibody at 1:100 dilution.Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Cytokeratin 2 antibody at 1:200 dilution.Application
Sample (30 ug of whole cell lysate) A: A431| Product Name | Cytokeratin 2 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cytokeratin 2 (keratin 2)The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cytokeratin 2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Dog, ChimpanzeeDog (81%), Chimpanzee (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CK-2e antibody K2e antibody KRT2A antibody KRT2E antibody KRTE antibody MGC116967 antibody MGC116968 antibody KRT2 antibody epidermal ichthyosis bullosa of Siemens antibody epithelial keratin-2e antibody keratin-2 epidermis antibody cytokeratin- |
|---|---|
| Molecular Weight(MW) | 65 kDa (note) |
Application
Immunohistochemical analysis of paraffin-embedded H661 xenograft, using KRT2 antibody at 1:100 dilution.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Cytokeratin 2 antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: A431| Positive Control | A431 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
