| Product Name | Cytokeratin 4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [6B10] to Cytokeratin 4Intermediate filaments are abundant cytoplasmic structural proteins in most vertebrate cells. Cytokeratins, a group comprised of at least 29 different proteins are characteristic of epithelial and trichocytic cells. Cytokeratin 4 is a member of the type II, neutralto- basic subfamily. It is a 59 kD polypeptide differentially expressed in various human tissues. Cytokeratin 4 can be detected by biochemical or immunohistochemical means in adult non-cornified squamous epithelium such as that of the superficial and intermediate cells of the esophagus, exocervix, tongue, vagina, larynx,pharynx, epiglottis, and anus as well as in the superficial cells of the cornea. In addition, its presence was demonstrated in suprabasal cells of the urinary bladder transitional epithelium, in single cells and cell groups of sweat glands and prostatic ducts and in cylindrical ciliated bronchial cells. Cytokeratin 4 may be detected in squamous cell carcinomas derived from several non-cornified stratified epithelia. Monoclonal anti-cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal anti-Cytokeratin 4 is a chain specific antibody which may facilitate typing of normal, metaplastic and neoplastic cells. |
| Immunogen | GTX11215 is derived from the 6B10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a cytokeratin preparation purified from human esophagus. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6B10 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| IF: Use at a dilution of 1/300. IHC-P: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects a band of approximately 59 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | DogPigRatCatGoatGuinea PigHamsterMammalian speciesRabbitSheep |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Cytokeratin 4 3851 CK4 CYK4 P19013 123940 KRT4 FLJ31692 K4 Cytokeratin4 |
|---|---|
| Target | Cytokeratin 4 |
| Tissue Specificity | reacts specifically with human non-cornified squamous epithelium (e.g. exocervix, esophagus, cornea). Ciliated pseudostratified epithelium of bronchi is weakly stained. Ducts of skin sweat glands and prostate are focally stained. Some endocervical columnar cells may also react with the antibody. |
| Application Notes | IF: Use at a dilution of 1/300. IHC-P: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects a band of approximately 59 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Raw ascites containing 0.1% sodium azide |
