| Product Name | Cytokeratin 5 + 14 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [16.4] to Cytokeratin 5+14Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis.Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells.Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Belonging to the type A (acidic) subfamily of low molecular weight keratins and existing in combination with keratin 5, keratin 14 distinguishes stratified epithelial cells from simple epithelial cells and is useful in identification of squamous cell carcinomas. It is considered a prognostic marker in breast carcinomas. |
| Immunogen | Tissue / cell preparation (cytokeratin enriched extract of feline tongue epithelium). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 16.4 |
| Host Species | Mouse |
| Tested Applications | IHC-FWB |
| IHC: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects bands of approximately 58 and 50 kDa corresponding to cytokeratins 5 and 14 respectively. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | 3852 Cytokeratin 5 + 14 3861 CK14 P02533 EBS3 CK5 KRT14 EBS4 Cytokeratin5 + 14 P13647 K5 EBS2 K14 KRT5 KRT5A 131800 |
|---|---|
| Target | Cytokeratin 5 + 14 |
| Cellular Localization | Basal layer |
| Application Notes | IHC: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects bands of approximately 58 and 50 kDa corresponding to cytokeratins 5 and 14 respectively. Optimal dilutions/concentrations should be determined by the end user.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.08% Sodium Azide. Constituents: PBS |
