Application
Cytokeratin 5 antibody detects Cytokeratin 5 protein at cell membrane and cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Cytokeratin 5 antibody diluted at 1:500.Application
Sample (30 µg of whole cell lysate) A: HCT116 7.5% SDS PAGE Cytokeratin 5 antibody diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of methanol-fixed HeLa, using Cytokeratin 5 antibody at 1:200 dilution.Application
Cytokeratin 5 antibody detects Cytokeratin 5 protein at cytosol and membrane on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Cytokeratin 5 antibody dilution: 1:500.Application
Cytokeratin 5 antibody detects Cytokeratin 5 protein at cytosol and membrane on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Cytokeratin 5 antibody dilution: 1:500.| Product Name | Cytokeratin 5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cytokeratin 5 (keratin 5)The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cytokeratin 5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Dog, Chicken, Chimpanzee, BovineRat (97%), Dog (97%), Chicken (80%), Chimpanzee (100%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CK5 antibody DDD antibody EBS2 antibody K5 antibody KRT5A antibody KRT5 antibody 58 kda cytokeratin antibody keratin type II cytoskeletal 5 antibody keratin 5 (epidermolysis bullosa simplex Dowling-Meara/Kobner/Weber-Cockayne types) antibody cyt |
|---|---|
| Molecular Weight(MW) | 62 kDa (note) (2) |
Application
Cytokeratin 5 antibody detects Cytokeratin 5 protein at cell membrane and cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Cytokeratin 5 antibody diluted at 1:500.
Application
Sample (30 µg of whole cell lysate) A: HCT116 7.5% SDS PAGE Cytokeratin 5 antibody diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using Cytokeratin 5 antibody at 1:200 dilution.
Application
Cytokeratin 5 antibody detects Cytokeratin 5 protein at cytosol and membrane on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Cytokeratin 5 antibody dilution: 1:500.
Application
Cytokeratin 5 antibody detects Cytokeratin 5 protein at cytosol and membrane on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Cytokeratin 5 antibody dilution: 1:500.| Positive Control | HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
