Application
Detection of Cytokeratin 7 by IHC in Human PancreasApplication
Formalin fixed paraffin embedded human skin stained with Cytokeratin 7 antibody (cat. # ).Application
Cytokeratin 7 (54 kd)Formalin fixed paraffin embedded human skin stained with Cytokeratin 7 (cat. # ) using ABC and AEC chromogen.Application
Formalin fixed paraffin embedded human lung stained with Cytokeratin 7 antibody .| Product Name | Cytokeratin 7 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Mouse Antibody to Human Cytokeratin 7This antibody recognizes keratin polypeptide of 54 kD. It stains a number of epithelial cells including ductal and glandular epithelia. Stratified squamous epithelia are positive but transitional epithelium of the urinary tract does not show any staining. This antibody labels adenocarcinomas and transitional carcinomas but does not react with squamous cell carcinomas. |
| Immunogen | BALB/C mice were immunized with ovarian carcinoma cell line, OTN11. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | OV-TL 12/30 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-P |
| This antibody may be diluted to a titer of 1:25-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion of paraffin embedded tissues is recommended prior to the immunostaining.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 3855 148059 K7 Cytokeratin7 MGC3625 Krt27 MGC129731 MGC11625 KRT7 Krt2-7 SCL K2C7 110310 Cytokeratin 7 P08729 CK7 D15Wsu77e |
|---|---|
| Target | Cytokeratin 7 |
| Cellular Localization | Cytoplasmic |
| Light Chain Type | Kappa |
Application
Detection of Cytokeratin 7 by IHC in Human Pancreas
Application
Formalin fixed paraffin embedded human skin stained with Cytokeratin 7 antibody (cat. # ).
Application
Cytokeratin 7 (54 kd)Formalin fixed paraffin embedded human skin stained with Cytokeratin 7 (cat. # ) using ABC and AEC chromogen.
Application
Formalin fixed paraffin embedded human lung stained with Cytokeratin 7 antibody .| Positive Control | Skin |
|---|---|
| Application Notes | This antibody may be diluted to a titer of 1:25-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion of paraffin embedded tissues is recommended prior to the immunostaining.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM249250