Application
Formalin fixed paraffin embedded human skin stained with Cytokeratin antibody.| Product Name | Cytokeratin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Mouse Antibody to Human CytokeratinThis antibody recognizes keratin polypeptide of 45 and 56 kD. It shows a broad pattern of reactivity with human epithelial tissues from simple glandular epithelia to stratified squamous epithelia. This antibody is of value in the identification of tumor cells as being of epithelial or less commonly of mesothelial origin. |
| Immunogen | BALB/C mice were immunized with human Keratin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | LP34 |
| Host Species | Mouse |
| Tested Applications | IHC-P |
| This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion of paraffin embedded tissues is recommended prior to the immunostaining.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EHK1 P04264 3848 CK1 KRT1A 113800 KRT1 K1 Cytokeratin |
|---|---|
| Target | Cytokeratin |
| Cellular Localization | Cytoplasmic |
| Light Chain Type | Kappa |
Application
Formalin fixed paraffin embedded human skin stained with Cytokeratin antibody.| Positive Control | Skin |
|---|---|
| Application Notes | This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion of paraffin embedded tissues is recommended prior to the immunostaining.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
