Monoclonal Mouse Antibody to Human CytokeratinThis antibody recognizes keratin polypeptide of 45, 46 and 56.5 kD. It shows a broad pattern of reactivity with human epithelial tissues from simple glandular epithelia to stratified squamous epithelia, like epidermis, mammary gland ducts and tracheal epithelium.
Immunogen
BALB/C mice were immunized with crude extract of splenic cells from a nude mouse engrafted with MCF-7 cells.
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
MNF116
Host Species
Mouse
Tested Applications
IHC-P
This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion of paraffin embedded tissues is recommended prior to the immunostaining.:
Formalin fixed paraffin embedded human tonsil stained with Cytokeratin antibody (cat. # ).
Positive Control
Skin
Application Notes
This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion of paraffin embedded tissues is recommended prior to the immunostaining.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution, aliquot and store at 4°C.
Storage Buffer
This product is supplied as a tissue culture supernatant and contains less than 0.1% sodium azide as a preservative.