Application
Formalin fixed paraffin embedded human skin stained with H.M.W. Cytokeratin antibody (cat. # )| Product Name | Cytokeratin high MW antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Mouse Antibody to Human Cytokeratin ( H.M.Weight )This antibody recognizes keratin polypeptide of 68, 58, 56.5 and 50 kD in extract of stratum corneum. The antibody reacts with squamous, ductal and other complex epithelia. It stains with adenocarcinomas, breast, pancreas, bile duct, salivary gland and transitional cell carcinomas. |
| Immunogen | BALB/c mice were immunized with solublized keratin extract from human stratum corneum. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 34ßE12 |
| Host Species | Mouse |
| Tested Applications | IHC-P |
| This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EHK1 P04264 3848 Cytokeratin high MW CK1 KRT1A 113800 KRT1 K1 |
|---|---|
| Target | Cytokeratin high MW |
| Cellular Localization | Cytoplasmic |
| Light Chain Type | kappa |
Application
Formalin fixed paraffin embedded human skin stained with H.M.W. Cytokeratin antibody (cat. # )| Positive Control | Skin |
|---|---|
| Application Notes | This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
