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D4 GDI antibody
Catalog Number:OM249348
OM249348
Discount Price
OM249348-100ul
1~2 Week
100ul
OM249348-50ul
50ul
1~2 Week
Product Profile
Product Name D4 GDI antibody
Antibody Type Primary Antibodies
Product description Mouse monoclonal [97A1015] to D4 GDID4 GDI belongs to the family of Rho GDIs or GDP dissociation inhibitors for the Rho/Rac family of small G proteins; consisting of RhoGDI 1, D4 GDI/RhoGDI2 and Rho GDI3. It inhibits the release of GDP from the Rho proteins, essential for GTP loading and activation of GTPase activity. D4 GDI is located in the cytoplasm and in involved in the regulation of the membrane association and dissociation cycle of Rho/Rac proteins. It is known to be a substrate for caspase 3 during Fas mediated apoptosis.
Immunogen Synthetic peptide corresponding to amino acid residue(s) in the Fas-induced cleavage site of human D4-GDI.
Key Feature
Clonality Monoclonal
Isotype IgG1
Clone Number 97A1015
Host Species Mouse
Tested Applications ELISAIHC-PWB
WB: Use at a concentration of 1 - 2 μg/ml. Detects a band of approximately 23 kDa. Optimal dilutions/concentrations should be determined by the end user.:
Species Reactivity Human
Concentration 0.5mg/ml
Purification Affinity purified
Target Information
Gene Synonyms Ly-GDI
D4 GDI
602843
397
P52566
RAP1GN1
GDIA2
D4
LyGDI
ARHGDIB
GDID4
Ly GDI
Target D4 GDI
Cellular Localization Cytoplasmic and Nuclear
Application

Application

Immunohistochemical analysis of D4GD1-cv in formalin-fixed, paraffin-embedded human breast tumor tissue using an isotype control (top) and IMG-160A (bottom) at 5 ug/ml

Application

Western blot analysis of D4GDI in Jurkat cells using at 2 ug/ml and IMG-160A at 0.1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. IMG-396A detects both the full-length and cleaved forms of D4GDI while IMG-160A specifically detects only the cleaved form of the protein. IMGENEX's goat anti-mouse Ig HRP conjugate (IMGENEX) was used for this test.

Application

HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the or D4-GDI antibodies (0.5 ug antibody/test/1 million cells) and Imgenex's Intracellular Staining Flow Assay Kit . A: IMG-160A which recognizes only cleaved D4-GDI. B: which recognizes both full-length and cleaved.
Positive Control HL60 cells treated with 10 ng/ml of an anti-Fas mAb
Application Notes WB: Use at a concentration of 1 - 2 μg/ml. Detects a band of approximately 23 kDa. Optimal dilutions/concentrations should be determined by the end user.:
Additional Information
Form Liquid
Storage Instructions Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
Storage Buffer PBS, containing 0.05% BSA and 0.05% sodium azide
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