Application
Immunohistochemical analysis of D4GD1-cv in formalin-fixed, paraffin-embedded human breast tumor tissue using an isotype control (top) and IMG-160A (bottom) at 5 ug/mlApplication
Western blot analysis of D4GDI in Jurkat cells using at 2 ug/ml and IMG-160A at 0.1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. IMG-396A detects both the full-length and cleaved forms of D4GDI while IMG-160A specifically detects only the cleaved form of the protein. IMGENEX's goat anti-mouse Ig HRP conjugate (IMGENEX) was used for this test.Application
HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the or D4-GDI antibodies (0.5 ug antibody/test/1 million cells) and Imgenex's Intracellular Staining Flow Assay Kit . A: IMG-160A which recognizes only cleaved D4-GDI. B: which recognizes both full-length and cleaved.| Product Name | D4 GDI antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [97A1015] to D4 GDID4 GDI belongs to the family of Rho GDIs or GDP dissociation inhibitors for the Rho/Rac family of small G proteins; consisting of RhoGDI 1, D4 GDI/RhoGDI2 and Rho GDI3. It inhibits the release of GDP from the Rho proteins, essential for GTP loading and activation of GTPase activity. D4 GDI is located in the cytoplasm and in involved in the regulation of the membrane association and dissociation cycle of Rho/Rac proteins. It is known to be a substrate for caspase 3 during Fas mediated apoptosis. |
| Immunogen | Synthetic peptide corresponding to amino acid residue(s) in the Fas-induced cleavage site of human D4-GDI. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 97A1015 |
| Host Species | Mouse |
| Tested Applications | ELISAIHC-PWB |
| WB: Use at a concentration of 1 - 2 μg/ml. Detects a band of approximately 23 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Ly-GDI D4 GDI 602843 397 P52566 RAP1GN1 GDIA2 D4 LyGDI ARHGDIB GDID4 Ly GDI |
|---|---|
| Target | D4 GDI |
| Cellular Localization | Cytoplasmic and Nuclear |
Application
Immunohistochemical analysis of D4GD1-cv in formalin-fixed, paraffin-embedded human breast tumor tissue using an isotype control (top) and IMG-160A (bottom) at 5 ug/ml
Application
Western blot analysis of D4GDI in Jurkat cells using at 2 ug/ml and IMG-160A at 0.1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. IMG-396A detects both the full-length and cleaved forms of D4GDI while IMG-160A specifically detects only the cleaved form of the protein. IMGENEX's goat anti-mouse Ig HRP conjugate (IMGENEX) was used for this test.
Application
HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the or D4-GDI antibodies (0.5 ug antibody/test/1 million cells) and Imgenex's Intracellular Staining Flow Assay Kit . A: IMG-160A which recognizes only cleaved D4-GDI. B: which recognizes both full-length and cleaved.| Positive Control | HL60 cells treated with 10 ng/ml of an anti-Fas mAb |
|---|---|
| Application Notes | WB: Use at a concentration of 1 - 2 μg/ml. Detects a band of approximately 23 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS, containing 0.05% BSA and 0.05% sodium azide |
Data sheet for OM249348