Application
Immunofluorescence of Dact2 in Human Brain cells with Dact2 antibody at 20 ug/mL.Application
Immunohistochemistry of Dact2 in human brain tissue with Dact2 antibody at 2.5 ug/ml.Application
Western blot analysis of Dact2 in SK-N-SH cell lysate with Dact2 antibody at 1 ug/ml.| Product Name | Dact2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Dact2The Wnt signaling cascade is a conserved process in multicellular animals that plays important roles during development and can contribute to cancer and other diseases. Many members of this pathway are also expressed in the postnatal tissues such as brain. One such protein is Dact2, a member of the Dact protein family that was initially identified through binding to Disheveled (Dvl), a cytoplasmic protein essential to Wnt signaling. Dact2 is most prominent during the development of the thymus kidneys, and salivary gland. Dact2 is thought to play a role distinct from that of Dact1 with Dact2 having a greater impact on a b-catenin-independent process termed planar cell polarity/convergent-extension signaling. Furthermore, Dact2 but not Dact1 can inhibit Nodal signaling by promoting the endocytic degradation of TGF-b receptors. At least two isoforms of Dact2 are known to exist. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Dact2 Dapper homolog2 Dapper homolog 2 Dapper antagonist of beta-catenin 2 Dapper antagonist of betacatenin2 Dapper antagonist of betacatenin 2 |
|---|---|
| Target | Dact2 |
| Tissue Specificity | This antibody is specific for Dact2 |
Application
Immunofluorescence of Dact2 in Human Brain cells with Dact2 antibody at 20 ug/mL.
Application
Immunohistochemistry of Dact2 in human brain tissue with Dact2 antibody at 2.5 ug/ml.
Application
Western blot analysis of Dact2 in SK-N-SH cell lysate with Dact2 antibody at 1 ug/ml.| Positive Control | Blocking Peptide: |
|---|---|
| Application Notes | ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.02% sodium azide |
