Application
Immunofluorescence analysis of methanol-fixed HeLa, using DAK antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded human lung SCC, using DAK antibody at 1:100 dilution.Application
Sample(30 µg whole cell lysate) A:Hep G2 B:Raji 7.5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | DAK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DAK (bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing))This gene is a member of the family of dihydroxyacetone kinases, which have a protein structure distinct from other kinases. The product of this gene phosphorylates dihydroxyacetone, and also catalyzes the formation of riboflavin 4',5'-phosphate (aka cyclin FMN) from FAD. Several alternatively spliced transcript variants have been identified, but the full-length nature of only one has been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DAK. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Chimpanzee, BovineMouse (82%), Rat (83%), Chimpanzee (100%), Bovine (89%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp586B1621 antibody MGC5621 antibody NET45 antibody DAK antibody ATP-dependent dihydroxyacetone kinase antibody DHA kinase antibody Dha kinase/FMN cyclase antibody bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing) antib |
|---|---|
| Molecular Weight(MW) | 59 kDa (note) |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using DAK antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human lung SCC, using DAK antibody at 1:100 dilution.
Application
Sample(30 µg whole cell lysate) A:Hep G2 B:Raji 7.5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HepG2 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
