Application
Immunocytochemical staining of ZIP kinase in Jurkat cells with ZIP kinase antibody at 10μg/ml.Application
Western blot analysis of ZIP kinase in HeLa (H) and Jurkat (J) cell lysates with ZIP kinase antibody at 1 g/ml.| Product Name | DAP Kinase 3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to DAP Kinase 3Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. A novel serine/threonine kinas that mediates apoptosis was recently identified and designated ZIP kinase (1). ZIP kinase contains an N-terminal kinase domain and a C-terminal leucine zipper structure and binds to ATF4 that is a member of ATF/CREB family. ZIP kinase has high sequence homology to DAP kinase (death-associated protein kinase), which is a mediator of apoptosis induced by gamma interferon. Overexpression of ZIP kinase induces apoptosis. ZIP and DAP kinases represent a novel kinase family, which mediates apoptosis through their catalytic activities. The messenger RNA was ubiquitously expressed in various tissues (1). |
| Immunogen | synthetic peptide corresponding to amino acids 279 to 298 of human origin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Western blot at 1:1000 dilution and a 52kDa band should be detected.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | O43293 603289 ZIPK 1613 ZIP Kinase DAPK3 ZIP FLJ36473 |
|---|---|
| Target | DAP Kinase 3 |
| Tissue Specificity | It has no cross reactivity to DAP kinase. |
| Cellular Localization | Nuclear |
Application
Immunocytochemical staining of ZIP kinase in Jurkat cells with ZIP kinase antibody at 10μg/ml.
Application
Western blot analysis of ZIP kinase in HeLa (H) and Jurkat (J) cell lysates with ZIP kinase antibody at 1 g/ml.| Positive Control | HeLa whole cell lysate or Jurkat whole cell lysate |
|---|---|
| Application Notes | Western blot at 1:1000 dilution and a 52kDa band should be detected.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: PBS |
