Application
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:500Application
DAP Kinase 3 antibody detects DAP Kinase 3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DAP Kinase 3 protein stained by DAP Kinase 3 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000.| Product Name | DAP Kinase 3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DAP kinase 3 (death-associated protein kinase 3)Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human DAP Kinase 3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Xenopus laevis, Rhesus Monkey, Chimpanzee, BovineMouse (96%), Xenopus laevis (87%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ36473 antibody ZIP antibody ZIPK antibody DAPK3 antibody DAP-like kinase antibody death-associated protein kinase 3 antibody ZIP-kinase antibody DAP kinase 3 antibody dlk antibody MYPT1 kinase antibody ZIP kinase isoform antibody zipper-inter |
|---|---|
| Molecular Weight(MW) | 53 kDa (note) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:500
Application
DAP Kinase 3 antibody detects DAP Kinase 3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DAP Kinase 3 protein stained by DAP Kinase 3 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000.| Positive Control | A549 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
