Application
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using DAP12 antibody at 1:500 dilution.Application
Western Blot analysis of TYROBP expression in transfected 293T cell line by TYROBP polyclonal antibody. A: Non-transfected lysate. B: TYROBP transfected lysate. 15% SDS PAGE diluted at 1:500| Product Name | DAP12 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DAP12 (TYRO protein tyrosine kinase binding protein)This gene encodes a transmembrane signaling polypeptide which contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. The encoded protein may associate with the killer-cell inhibitory receptor (KIR) family of membrane glycoproteins and may act as an activating signal transduction element. This protein may bind zeta-chain (TCR) associated protein kinase 70kDa (ZAP-70) and spleen tyrosine kinase (SYK) and play a role in signal transduction, bone modeling, brain myelination, and inflammation. Mutations within this gene have been associated with polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL), also known as Nasu-Hakola disease. Its putative receptor, triggering receptor expressed on myeloid cells 2 (TREM2), also causes PLOSL. Two alternative transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described, but their full-length nature has not been deterimined. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human DAP12. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Dog, Rhesus Monkey, BovineDog (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DAP12 antibody KARAP antibody PLOSL antibody TYROBP antibody KAR-associated protein antibody killer-activating receptor-associated protein antibody DNAX-activation protein 12 antibody killer activating receptor associated protein antibody TYRO prot |
|---|---|
| Molecular Weight(MW) | 12 kDa (note) (4) |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
Application
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using DAP12 antibody at 1:500 dilution.
Application
Western Blot analysis of TYROBP expression in transfected 293T cell line by TYROBP polyclonal antibody. A: Non-transfected lysate. B: TYROBP transfected lysate. 15% SDS PAGE diluted at 1:500| Positive Control | DAP12 tansfected 293T cell |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
