Application
Immunofluorescence analysis of methanol-fixed HeLa, using DAP3 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded MDA-MB231 xenograft, using DAP3 antibody at 1:250 dilution.Application
Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE DAP3 antibody diluted at 1:1000| Product Name | DAP3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DAP3 (death associated protein 3)Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Splice variants that differ in the 5' UTR have been found for this gene; both variants encode the same protein. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DAP3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (85%), Rat (87%), Bovine (89%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DAP-3 antibody DKFZp686G12159 antibody FLJ12817 antibody MGC126058 antibody MGC126059 antibody MRP-S29 antibody MRPS29 antibody bMRP-10 antibody DAP3 antibody ionizing radiation resistance conferring protein antibody death-associated protein 3 an |
|---|---|
| Molecular Weight(MW) | 46 kDa (note) (5) |
| Cellular Localization | Mitochondrion |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using DAP3 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded MDA-MB231 xenograft, using DAP3 antibody at 1:250 dilution.
Application
Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE DAP3 antibody diluted at 1:1000| Positive Control | A549 , HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
