Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using EIF4G2 antibody at 1:100 dilution.Application
DAP5 antibody detects DAP5 protein at cytosol on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. DAP5 antibody dilution: 1:500.Application
Immunohistochemical analysis of paraffin-embedded human skeletal muscle , using DAP5 antibody(10 μg/ml).Application
Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE diluted at 1:500Application
DAP5 antibody detects EIF4G2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: EIF4G2 protein stained by DAP5 antibody diluted at 1:500. Blue: Hoechst 33343 staining.Application
Sample(30 μg of whole cell lysate) A:A431 7.5% SDS PAGE diluted at 1:1000| Product Name | DAP5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DAP5 (eukaryotic translation initiation factor 4 gamma, 2)Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human DAP5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Rabbit, Chicken, BovineRat (100%), Rabbit (100%), Chicken (90%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AAG1 antibody DAP5 antibody FLJ41344 antibody NAT1 antibody P97 antibody EIF4G2 antibody death-associated protein 5 antibody eukaryotic translation initiation factor 4G-like 1 antibody DAP-5 antibody eukaryotic translation initiation factor 4 gamm |
|---|---|
| Molecular Weight(MW) | 102 kDa (note) (3) |
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using EIF4G2 antibody at 1:100 dilution.
Application
DAP5 antibody detects DAP5 protein at cytosol on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. DAP5 antibody dilution: 1:500.
Application
Immunohistochemical analysis of paraffin-embedded human skeletal muscle , using DAP5 antibody(10 μg/ml).
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE diluted at 1:500
Application
DAP5 antibody detects EIF4G2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: EIF4G2 protein stained by DAP5 antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Application
Sample(30 μg of whole cell lysate) A:A431 7.5% SDS PAGE diluted at 1:1000| Positive Control | A431 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
