| Product Name | Daxx antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [DAXX-03] to DaxxDaxx is an apoptosis-modulating death-domain associated protein with functions in transcriptional regulation. Daxx functions bot in cytoplasm, where it interacts with Fas, and in nucleus (residing in PML oncogenic domains), where it is involved in SUMO-dependent regulation of transcription and subnuclear compartmentalization. Daxx senzitizes the cells to apoptosis, but on the other hand, this protein may also serve in preventing apoptosis in the early embryo. Even regarding the transcription, Daxx can serve both as a corepressor and a coactivator. During ischemic stress, Daxx translocates from the nucleus to the cytoplasm, where in regulates sodium hydrogen exchanger isoform 1 (NHE1).Entrez Gene: 6p21.3 |
| Immunogen | His6-tagged protein corresponding to amino acids 580-740 of human Daxx. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | DAXX-03 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| WB: Use at 2 µg/mL: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BING2 DAP6 MGC126245 MGC126246 |
|---|---|
| Target | Daxx |
| Tissue Specificity | The antibody DAXX-03 reacts with C-terminal part of Daxx, a 110 kDa intracellular protein. |
| Cellular Localization | Dispersed throughout the nucleoplasm, in PML/POD/ND10 nuclear bodies, and in nucleoli. Colocalizes with a subset of interphase centromeres, but is absent from mitotic centromeres. Detected in cytoplasmic punctate structures. Translocates from the nucleus |
| Application Notes | WB: Use at 2 µg/mL: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
