Application
Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Raji 7.5% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Daxx antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Daxx antibody at 1:500 dilution.| Product Name | Daxx antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Daxx (death-domain associated protein)This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Daxx. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (86%), Rat (88%), Bovine (93%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BING2 antibody DAP6 antibody EAP1 antibody MGC126245 antibody MGC126246 antibody DAXX antibody CENP-C binding protein antibody death domain-associated protein 6 antibody ETS1-associated protein 1 antibody death-associated protein 6 antibody Fas-b |
|---|---|
| Molecular Weight(MW) | 81 kDa (note) (3) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Raji 7.5% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Daxx antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Daxx antibody at 1:500 dilution.| Positive Control | A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
