Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE diluted at 1:500Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DAZ3 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using DAZ3 antibody at 1:250 dilution.| Product Name | DAZ3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DAZ3 (deleted in azoospermia 3)This gene is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains one copy of the 10.8 kb repeat. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DAZ3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC126441 antibody pDP1679 antibody DAZ3 antibody deleted in azoospermia protein 3 antibody deleted in azoospermia 3 antibody |
|---|---|
| Molecular Weight(MW) | 55 kDa (note) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE diluted at 1:500
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DAZ3 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using DAZ3 antibody at 1:250 dilution.| Positive Control | HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
