Application
DAZL antibody detects DAZL protein by western blot analysis. Whole cell extracts (30 μg) and Mouse tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DAZL antibody diluted at a dilution of 1:1000.| Product Name | DAZL antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to DAZL (deleted in azoospermia-like)The DAZ (Deleted in AZoospermia) gene family encodes potential RNA binding proteins that are expressed in prenatal and postnatal germ cells of males and females. The protein encoded by this gene is localized to the nucleus and cytoplasm of fetal germ cells and to the cytoplasm of developing oocytes. In the testis, this protein is localized to the nucleus of spermatogonia but relocates to the cytoplasm during meiosis where it persists in spermatids and spermatozoa. Transposition and amplification of this autosomal gene during primate evolution gave rise to the DAZ gene cluster on the Y chromosome. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human DAZL. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DAZH antibody DAZL1 antibody DAZLA antibody MGC26406 antibody SPGYLA antibody DAZL antibody germline specific RNA binding protein antibody deleted in azoospermia-like 1 antibody SPGY-like-autosomal antibody deleted in azoospermia-like antibody DA |
|---|---|
| Target | DAZL |
| Molecular Weight(MW) | 33 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
DAZL antibody detects DAZL protein by western blot analysis. Whole cell extracts (30 μg) and Mouse tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DAZL antibody diluted at a dilution of 1:1000.| Positive Control | NT2D1 , mouse testis |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
