Application
Western blot showing detection of 0.1 µg of recombinant DbpB protein. Lane 1: Molecular weight markers. Lane 2: MBP-DbpB fusion protein (arrow; expected MW = 60.3 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).| Product Name | Dbp-B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Dbp-BDecorin-binding protein B, or DbpB, binds to decorin, which may mediate the adherence of B.burgdorferi to collagen fibers in skin and other tissues. Spirochetal surface adhesions mediate attachment to decorin, a major component of the host extracellular matrix enabling bacteria to colonize in mammalian tissues. The spirochete migrates from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH, and nutrients) and/or in coordination with the course of infection of the mammal host. Borrelia burgdorferi can colonize multiple tissues, and is capable of attachment to diverse cell types. The expression of decorin-binding protein (Dbp) A and/or DbpB, two B. burgdorferi surface proteins that bind GAGs, is sufficient to convert a high-passage nonadherent B. burgdorferi strain into one that efficiently binds 293 epithelial cells. |
| Immunogen | MBP-fusion protein corresponding to Borrelia burgdorferi Dbp-B protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | DBPB |
|---|---|
| Target | Dbp-B |
Application
Western blot showing detection of 0.1 µg of recombinant DbpB protein. Lane 1: Molecular weight markers. Lane 2: MBP-DbpB fusion protein (arrow; expected MW = 60.3 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Store vial at 4° C for short-term use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
| Storage Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide |
