Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DC8 antibody at 1:200 dilution.Application
DC8 antibody detects NSL1 protein by Western blot analysis. A. 30 µg C8D30 whole cell lysate/extract 12 % SDS-PAGE DC8 antibody dilution: 1:1000Application
Sample(30 μg of whole cell lysate) A:Hep G2 12% SDS PAGE diluted at 1:1000Application
DC8 antibody detects NSL1 protein by Western blot analysis. A. 30 µg Rat2 whole cell lysate/extract 12 % SDS-PAGE DC8 antibody dilution: 1:1000| Product Name | DC8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DC8 (NSL1, MIND kinetochore complex component, homolog (S. cerevisiae))This gene encodes a protein with two coiled-coil domains that localizes to kinetochores, which are chromosome-associated structures that attach to microtubules and mediate chromosome movements during cell division. The encoded protein is part of a conserved protein complex that includes two chromodomain-containing proteins and a component of the outer plate of the kinetochore. This protein complex is proposed to bridge centromeric heterochromatin with the outer kinetochore structure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DC8. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | BovineBovine (87%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | C1orf48 antibody DKFZp566O1646 antibody MIS14 antibody NSL1 antibody kinetochore-associated protein NSL1 homolog antibody NSL1 MIND kinetochore complex component homolog (S. cerevisiae) antibody |
|---|---|
| Molecular Weight(MW) | 32 kDa (note) |
| Cellular Localization | Nucleus , Kinetochore |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DC8 antibody at 1:200 dilution.
Application
DC8 antibody detects NSL1 protein by Western blot analysis. A. 30 µg C8D30 whole cell lysate/extract 12 % SDS-PAGE DC8 antibody dilution: 1:1000
Application
Sample(30 μg of whole cell lysate) A:Hep G2 12% SDS PAGE diluted at 1:1000
Application
DC8 antibody detects NSL1 protein by Western blot analysis. A. 30 µg Rat2 whole cell lysate/extract 12 % SDS-PAGE DC8 antibody dilution: 1:1000| Positive Control | HepG2 , C8D30 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
