Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DCAMKL2 antibody [N2C2], Internal diluted at 1:500.Application
DCAMKL2 antibody [N2C2], Internal detects DCAMKL2 protein at Cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in -20℃ 100% MeOH for 5 min. Green: DCAMKL2 protein stained by DCAMKL2 antibody [N2C2], Internal diluted at 1:500. Blue: Hoechst 33343 staining.| Product Name | DCAMKL2 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to DCAMKL2 (doublecortin-like kinase 2)This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. Mouse studies show that the DCX gene, another family member, and this gene share function in the establishment of hippocampal organization and that their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DCAMKL2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, RatMouse (94%), Rat (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CL2 antibody CLICK-II antibody CLICK2 antibody CLIK2 antibody DCAMKL2 antibody DCDC3 antibody DCDC3B antibody DCK2 antibody DKFZp761I032 antibody MGC45428 antibody DCLK2 antibody doublecortin and CaM kinase-like 2 antibody doublecortin-like and |
|---|---|
| Target | DCAMKL2 |
| Molecular Weight(MW) | 84 kDa (note) |
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DCAMKL2 antibody [N2C2], Internal diluted at 1:500.
Application
DCAMKL2 antibody [N2C2], Internal detects DCAMKL2 protein at Cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in -20℃ 100% MeOH for 5 min. Green: DCAMKL2 protein stained by DCAMKL2 antibody [N2C2], Internal diluted at 1:500. Blue: Hoechst 33343 staining.| Positive Control | A549 , H1299 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
