Application
Immunohistochemistry of DLCK1 in rat brain tissue with DLCK1 antibody at 2.5 ug/ml.Application
Western blot analysis of DCLK1 in human brain tissue lysate with DCLK1 antibody at (A) 0.5 and (B) 1 ug/ml.| Product Name | DCLK1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to DCLK1DCLK1 is one of three doublecortin-like kinases similar to the Ca2+/calmodulin-dependent protein kinase (CaMK) family. DCLK1 mRNA, like that of the homologous DCLK2 and DCLK3, is highly expressed in adult brain, but only DCLK1 and DCLK2 transcripts are present in human fetal brain and the developing mouse embryo, suggesting that DCLK1 and DCLK2 may play roles in cortical development. The DCLK proteins are homologous to Doublecortin (DCX), a gene that is mutated in X-linked human lissencephaly. In mouse models where the DCX gene has been disrupted, DCLK1 expression increases slightly and appears to compensate for the loss of DCX, as mice mutant for both DCX and DCLK1 show a severe phenotype including perinatal lethality, disorganized neocortical layering, and profound hippocampal cytoarchitectural disorganization. Unlike DCLK1, DCLK2 expression does not change in DCX-null mice. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DCDC3A DCLK1 Doublecortin kinase-like 1 Doublecortin kinaselike 1 DCAMKL1 Doublecortin kinaselike1 CLICK1 CL1 Doublecortin kinase like 1 |
|---|---|
| Target | DCLK1 |
| Tissue Specificity | This antibody is specific for DCLK1 |
Application
Immunohistochemistry of DLCK1 in rat brain tissue with DLCK1 antibody at 2.5 ug/ml.
Application
Western blot analysis of DCLK1 in human brain tissue lysate with DCLK1 antibody at (A) 0.5 and (B) 1 ug/ml.| Positive Control | Blocking Peptide: Cat.No. GTX85008-PEP - DCLK1 Peptide Lysates: Cat. No. GTX27918 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Antibody is supplied in PBS containing 0.02% sodium azide |
