Application
Immunofluorescence of DLCK3 in human brain tissue with DLCK3 antibody at 20 ug/ml.Application
Western blot analysis of DCLK3 in K562 cell lysate with DCLK3 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | DCLK3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to DCLK3DCLK3 is one of three doublecortin-like kinases similar to the Ca2+/calmodulin-dependent protein kinase (CaMK) family. DCLK3 mRNA, like that of the homologous DCLK1 and DCLK3, is highly expressed in adult brain, but only DCLK3 transcripts are present in liver and kidney, suggesting that DCLK3 may play other roles than in cortical development. The DCLK proteins are homologous to Doublecortin (DCX), a protein that is mutated in X-linked human lissencephaly. In mouse models where the DCX gene has been disrupted, DCLK1 expression increases slightly and appears to compensate for the loss of DCX, as mice mutant for both DCX and DCLK1 show a severe phenotype including perinatal lethality, disorganized neocortical layering, and profound hippocampal cytoarchitectural disorganization. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DCDC3C Doublecortin kinase-like 3 DCLK3 DCK32 Doublecortin kinaselike 3 CLr DCAMKL3 Doublecortin kinaselike3 Doublecortin kinase like 3 |
|---|---|
| Target | DCLK3 |
| Tissue Specificity | This antibody is specific for DCLK3 |
Application
Immunofluorescence of DLCK3 in human brain tissue with DLCK3 antibody at 20 ug/ml.
Application
Western blot analysis of DCLK3 in K562 cell lysate with DCLK3 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | Blocking Peptide: |
|---|---|
| Application Notes | ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.02% sodium azide |
