Application
Immunofluorescence of DLCK3 in human brain tissue with DLCK3 antibody at 20 μg/mL.Application
Immunohistochemistry of DCLK3 in mouse brain tissue with DCLK3 Antibodyat 5 μg/mL.Application
Immunofluorescence of DCLK3 in mouse brain tissue with DCLK3 Antibodyat 20 μg/mL.Application
Western blot analysis of DCLK3 in K562 cell lysate with DCLK3 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | DCLK3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | DCLK3 Antibody: DCLK3 is one of three doublecortin-like kinases similar to the Ca2+/calmodulin-dependent protein kinase (CaMK) family. DCLK3 mRNA, like that of the homologous DCLK1 and DCLK3, is highly expressed in adult brain, but only DCLK3 transcripts are present in liver and kidney, suggesting that DCLK3 may play other roles than in cortical development. The DCLK proteins are homologous to Doublecortin (DCX), a protein that is mutated in X-linked human lissencephaly. In mouse models where the DCX gene has been disrupted, DCLK1 expression increases slightly and appears to compensate for the loss of DCX, as mice mutant for both DCX and DCLK1 show a severe phenotype including perinatal lethality, disorganized neocortical layering, and profound hippocampal cytoarchitectural disorganization.1) Ohmae S, Takemoto-Kimura S, Okamura M, et al. Molecular identification and characterization of a family of kinases with homology to Ca2+/calmodulin-dependent protein kinases I/IV. J. Biol. Chem.2006; 281:20427-39. |
| Immunogen | DCLK3 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human DCLK3. The immunogen is located within the last 50 amino acids of DCLK3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| DCLK3 antibody can be used for detection of DCLK3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DCLK3 |
|---|---|
| Alternative Names | DCLK3 Antibody: CLR DCK3 DCDC3C DCAMKL3 KIAA1765 Doublecortin domain-containing protein 3C |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunofluorescence of DLCK3 in human brain tissue with DLCK3 antibody at 20 μg/mL.
Application
Immunohistochemistry of DCLK3 in mouse brain tissue with DCLK3 Antibodyat 5 μg/mL.
Application
Immunofluorescence of DCLK3 in mouse brain tissue with DCLK3 Antibodyat 20 μg/mL.
Application
Western blot analysis of DCLK3 in K562 cell lysate with DCLK3 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | DCLK3 antibody can be used for detection of DCLK3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | DCLK3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | DCLK3 Antibody is supplied in PBS containing 0.02% sodium azide. |
