Application
Western blot detection of DC-SIGN fusion protein in human placenta at (A) 1 and (B) 2 μg/mL.Application
Immunohistochemistry of DC-SIGN in lymph node tissue with DC-SIGN antibody at 5 μg/mL.| Product Name | DC-SIGN antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody to DC-SIGN [5D7]Dendritic cells (DCs) that control immune responses were recently found to capture and transport HIV from the mucosal area to remote lymph nodes (1), where DCs hand over HIV to CD4+ T lymphocytes. DCs also amplify the amount of virus and extend the duration of viral infectivity. Multiple strains of HIV-1, HIV-2 and SIV bind to DCs via DC-SIGN (2). ICAM-3 is the natural ligand for DC-SIGN (3). A DC-SIGN homologue (termed DC-SIGNR, L-SIGN, and DC-SIGN2) was identified recently (4-8). DC-SIGN forms a novel gene family with DC-SIGNR and many alternatively spliced isoforms of DC-SIGN and DC-SIGNR (8). The expression of DC-SIGN was found in mucosal tissues including placenta, small intestine, and rectum. |
| Immunogen | A recombinant His-tagged protein fragment corresponding to the extracellular region of human DC-SIGN. |
| Clonality | Monoclonal |
|---|---|
| Clone Number | 5D7 |
| Host Species | Mouse |
| Tested Applications | ELISAIHC-PWB |
| IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Dendritic cell-specific ICAM-3-grabbing nonintegrin 1 DCSIGN (5D7) DC-SIGN (5D7) Dendritic cellspecific ICAM3grabbing nonintegrin1 Dendritic cellspecific ICAM3grabbing nonintegrin 1 DC SIGN DCSIGN DC SIGN (5D7) |
|---|---|
| Target | DC-SIGN |
| Tissue Specificity | This antibody is specific for DC-SIGN |
Application
Western blot detection of DC-SIGN fusion protein in human placenta at (A) 1 and (B) 2 μg/mL.
Application
Immunohistochemistry of DC-SIGN in lymph node tissue with DC-SIGN antibody at 5 μg/mL.| Positive Control | Lysates: Cat. No. GTX15363 - Human Lymph Node Tissue Lysate |
|---|---|
| Application Notes | IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Antibody is supplied in PBS containing 0.02% sodium azide |
