Application
FACS analysis of human DC-SIGN transfected K562 cells using DC-SIGN antibody [MR-1].| Product Name | DC-SIGN antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [MR-1] to DC-SIGNThis gene encodes a transmembrane receptor and is often referred to as DC-SIGN because of its expression on the surface of dendritic cells and macrophages. The encoded protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses with a large impact on public health. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. Variations in the number of 23 amino acid repeats in the neck domain of this protein are rare but have a significant i |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MR-1 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IF |
| Flow Cytometry: 1/50 - 1/100 Optimal dilution / concentration should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CD209 DC-SIGN1 CLEC4L CDSIGN |
|---|---|
| Target | CD209 |
| Tissue Specificity | This antibody recognizes human DC-specific ICAM-3 grabbing nonintegrin (DC-SIGN). Clone MR-1 is reported to partially block the functional activity of DC-SIGN (Melero et al.). |
Application
FACS analysis of human DC-SIGN transfected K562 cells using DC-SIGN antibody [MR-1].| Application Notes | Flow Cytometry: 1/50 - 1/100 Optimal dilution / concentration should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | Phosphate buffered saline containing 0.09% Sodium Azide |
