Application
Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE diluted at 1:3000Application
Immunofluorescence analysis of methanol-fixed MCF-7, using Dynactin 2 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Dynactin 2 antibody at 1:250 dilution.| Product Name | DCTN2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DCTN2 (dynactin 2 (p50))This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DCTN2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Chimpanzee, BovineMouse (96%), Rat (96%), Xenopus laevis (82%), Chimpanzee (99%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DCTN50 antibody DYNAMITIN antibody RBP50 antibody DCTN2 antibody dynactin subunit 2 antibody dynactin complex 50 kDa subunit antibody 50 kD dynein-associated polypeptide antibody dynactin complex 50 kD subunit antibody 50 kDa dynein-associated poly |
|---|---|
| Molecular Weight(MW) | 44 kDa (note) (4) |
| Cellular Localization | Cytoplasm , cytoskeleton , Membrane |
Application
Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE diluted at 1:3000
Application
Immunofluorescence analysis of methanol-fixed MCF-7, using Dynactin 2 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Dynactin 2 antibody at 1:250 dilution.| Positive Control | MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
