Application
Sample (30 ug of whole cell lysate) A: A431 B: JurKat C: Raji 12% SDS PAGE diluted at 1:1000| Product Name | DCUN1D1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to DCUN1D1 (DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae))Part of an E3 ubiquitin ligase complex for neddylation. Required for neddylation of cullin components of E3 cullin-RING ubiquitin ligase complexes by enhancing the rate of cullins neddylation. Functions to recruit the NEDD8-charged E2 enzyme to the cullin component. Involved in the release of inhibitory effets of CAND1 on cullin-RING ligase E3 complex assembly and activity. Acts also as an oncogene facilitating malignant transformation and carcinogenic progression. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DCUN1D1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Zebrafish, Xenopus laevis, Chicken, Rhesus Monkey, BovineMouse (99%), Zebrafish (92%), Xenopus laevis (99%), Chicken (99%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DCUN1L1 antibody RP42 antibody SCCRO antibody SCRO antibody Tes3 antibody DCUN1D1 antibody RP42 homolog antibody DCUN1 domain-containing protein 1 antibody DCN1-like protein 1 antibody squamous cell carcinoma-related oncogene antibody defective i |
|---|---|
| Target | DCUN1D1 |
| Molecular Weight(MW) | 30 kDa (note) (3) |
Application
Sample (30 ug of whole cell lysate) A: A431 B: JurKat C: Raji 12% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
