Application
ApplicationApplication
Detection of Human DDB1 in (1) Jurkat, (2) MCF7, and (3) T47D cell lysates using GeneTex polyclonal antibody| Product Name | DDB1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal to Human DDB1 (C-terminus)The DNA damage-binding protein from HeLa cells is associated with polypeptides of relative mass 124,000 (DDB1) and 41,000 (DDB2) as determined by SDS-polyacrylamide gels. To test whether the DNA-repair defect in the subset of XPE patients that lack DNA damage-binding activity is caused by a defect in DDB, Keeney et al. (1994) injected purified human DDB protein into XPE cells. The injected DDB protein stimulated DNA repair to normal levels in those strains that lacked the DDB activity but did not stimulate repair in cells from XPE patients that contained the activity. These results provided direct evidence that defective DDB activity causes the repair defect in a subset of XPE patients and establishes a role for this activity in nucleotide-excision repair in vivo. It remains for mutation analysis to demonstrate whether the defect in XPE patients is in the DDB1 gene or the DDB2 gene. |
| Immunogen | Synthetic peptide conugated to protein carrier. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB: Use at an assay dependent dilution. Predicted molecular weight: 127 kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | XPEBF Q16531 UVDDB1 XAP1 XPE-BF UV-DDB1 DDB1 XPE DDBA 600045 XPCE 1642 XPE BF UV DDB1 |
|---|---|
| Target | DDB1 |
Application
Application
Application
Detection of Human DDB1 in (1) Jurkat, (2) MCF7, and (3) T47D cell lysates using GeneTex polyclonal antibody| Positive Control | T47D and MCF-7 whole cell lysate |
|---|---|
| Application Notes | For WB: Use at an assay dependent dilution. Predicted molecular weight: 127 kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing no preservatives |
