Application
Whole extract of human HEK-293T cells over-expressing C-Terminal Flag tagged fusion protein was separated on SDS-PAGE and probed with Rabbit Anti-FLAG antibody. The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. 0.4 μg/mL antibody 2. Negative Control: without first antibodyApplication
Whole extract of human HEK-293T cells over-expressing N-Terminal Flag tagged fusion protein was separated on SDS-PAGE and probed with Rabbit Anti-FLAG antibody. The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. 0.4 μg/mL antibody 2. Negative Control: without first antibodyApplication
Human HEK-293T cells over-expressing C-Terminal Flag tagged fusion protein were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 0.8 μg/mL Rabbit Anti-FLAG antibody. The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.Application
Human HEK-293T cells over-expressing N-Terminal Flag tagged fusion protein were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1.6 μg/mL Rabbit Anti-FLAG antibody. The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.| Product Name | DDDDK tag antibody |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Rabbit polyclonal antibody to DDDDK tagEpitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins. |
| Immunogen | aa CDYKDDDDK-GCKLH |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| This antibody recognizes the FLAG epitope located on FLAG-tagged fusion proteins at the N-terminus or C-terminus,applying dot blot, immunoblotting, immunoprecipitation and immunocytochemistry assays. WB 2.5 microgram/ml using E.Coli periplasmic extract expressing N-terminal FLAG-fusion protein (2Hrs RT) Indirect Immunofluorescence 5 micrograms/ml using 293T cells transfected with a plasmid encoding FLAG-JNK.: |
|
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Target | DDDDK tag |
|---|---|
| Tissue Specificity | The DDDDK epitope is an expression tag commonly used in protein expression expts. When compared to its corresponding native protein, a DDDDK-tagged rotein migrates at a larger size on SDS-PAGE, which is equivalent to the size of the epitope tag. The size of the FLAG epitope may vary depending on the expression vector being utilized, but usually range fom 3-5 kD. |
Application
Whole extract of human HEK-293T cells over-expressing C-Terminal Flag tagged fusion protein was separated on SDS-PAGE and probed with Rabbit Anti-FLAG antibody. The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. 0.4 μg/mL antibody 2. Negative Control: without first antibody
Application
Whole extract of human HEK-293T cells over-expressing N-Terminal Flag tagged fusion protein was separated on SDS-PAGE and probed with Rabbit Anti-FLAG antibody. The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. 0.4 μg/mL antibody 2. Negative Control: without first antibody
Application
Human HEK-293T cells over-expressing C-Terminal Flag tagged fusion protein were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 0.8 μg/mL Rabbit Anti-FLAG antibody. The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Application
Human HEK-293T cells over-expressing N-Terminal Flag tagged fusion protein were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1.6 μg/mL Rabbit Anti-FLAG antibody. The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.| Application Notes | This antibody recognizes the FLAG epitope located on FLAG-tagged fusion proteins at the N-terminus or C-terminus,applying dot blot, immunoblotting, immunoprecipitation and immunocytochemistry assays. WB 2.5 microgram/ml using E.Coli periplasmic extract expressing N-terminal FLAG-fusion protein (2Hrs RT) Indirect Immunofluorescence 5 micrograms/ml using 293T cells transfected with a plasmid encoding FLAG-JNK.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 10 mM phosphate, 150 mM NaCl, pH 7.4, containing 1% BSA and 0.05% sodium azide. |
