Application
An ELISA plate is coated with 50 μL of a DDDDK tag conjugated BSA at concentration of 10 μg/mL. The peptide conjugated carrier protein is coated and detected with anti-DDDDK tag antibody at concentration ranged from 1.95 to 125 ng/mL.Application
DDDDK tag antibody detects DDDDK tag at cytoplasm and nucleus by immunofluorescent analysis. Samples: HeLa cells mock (left) and transfected with ZNF365–fusion protein (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: DDDDK tag stained by DDDDK tag antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Western blot analysis of extracts from untransfected control cells and transfected cells overexpressing DDDDK-tagged protein (~27 kDa), using DDDDK tag antibody. A: Transfected lysate (10 µg). B: Non-transfected lysate (10 µg). C: Non-transfected lysate (30 µg). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | DDDDK tag antibody |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Rabbit Polyclonal antibody to DDDDK tag |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAFACSICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanOther |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DDDDK tag antibody, DDDDK epitope tag antibody, DYKDDDDK epitope tag antibody, DYKDDDDK tag antibody, ECS epitope tag antibody, ECS tag antibody, Enterokinase Cleavage Site epitope tag antibody, Flag tag antibody |
|---|
Application
An ELISA plate is coated with 50 μL of a DDDDK tag conjugated BSA at concentration of 10 μg/mL. The peptide conjugated carrier protein is coated and detected with anti-DDDDK tag antibody at concentration ranged from 1.95 to 125 ng/mL.
Application
DDDDK tag antibody detects DDDDK tag at cytoplasm and nucleus by immunofluorescent analysis. Samples: HeLa cells mock (left) and transfected with ZNF365–fusion protein (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: DDDDK tag stained by DDDDK tag antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Western blot analysis of extracts from untransfected control cells and transfected cells overexpressing DDDDK-tagged protein (~27 kDa), using DDDDK tag antibody. A: Transfected lysate (10 µg). B: Non-transfected lysate (10 µg). C: Non-transfected lysate (30 µg). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | Transfected lysate |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
