Application
An ELISA plate is coated with 50 μL of a DDDDK tag conjugated BSA at concentration of 10 μg/mL. The peptide conjugated carrier protein is coated and detected with anti-DDDDK tag antibody at concentration ranged from 1.95 to 125 ng/mL.Application
DDDDK tag antibody detects DDDDK tag at cytoplasm and nucleus by immunofluorescent analysis. Samples: HeLa cells mock (left) and transfected with ZNF365–fusion protein (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: DDDDK tag stained by DDDDK tag antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | DDDDK tag antibody |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Rabbit polyclonal antibody to DDDDK tagEpitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins. |
| Immunogen | Rabbits were immunized with xxxDDDDK-peptide conjugated to KLH. After multiple immunizations in Freund's adjuvant, serum was collected and the antibodies were affinity purified using the peptide immobilized on a solid phase. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| For ELISA: Use at a dilution of 1:5,000-1:20,000. For ICC/IF: Use at a dilution of 1:100-1:1000. For WB: Use at a dilution of 1:1000-1:10000. Optimal dilutions/concentrations should be determined by the researcher. | |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Target | DDDDK tag |
|---|
Application
An ELISA plate is coated with 50 μL of a DDDDK tag conjugated BSA at concentration of 10 μg/mL. The peptide conjugated carrier protein is coated and detected with anti-DDDDK tag antibody at concentration ranged from 1.95 to 125 ng/mL.
Application
DDDDK tag antibody detects DDDDK tag at cytoplasm and nucleus by immunofluorescent analysis. Samples: HeLa cells mock (left) and transfected with ZNF365–fusion protein (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: DDDDK tag stained by DDDDK tag antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Application Notes | For ELISA: Use at a dilution of 1:5,000-1:20,000. For ICC/IF: Use at a dilution of 1:100-1:1000. For WB: Use at a dilution of 1:1000-1:10000. Optimal dilutions/concentrations should be determined by the researcher. |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
