Application
Western blot analysis of extracts from JK cells (Lane 2), using DDR1 (Phospho-Tyr513) Antibody. The lane on the left is treated with antigen-specific peptide.Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using DDR1 (Phospho-Tyr513) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | DDR1 (phospho Tyr513) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DDR1 (phospho Tyr513)Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene belongs to a subfamily of tyrosine kinase receptors with homology to Dictyostelium discoideum protein discoidin I in their extracellular domain, and that are activated by various types of collagen. Expression of this protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2011] |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 513 (K-K-Y(p)-V-R) derived from Human DDR1 . |
| Modification | p-Tyr513 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:1000. IHC: 1:50 - 1:100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | discoidin domain receptor tyrosine kinase 1 , EDDR1 , PTK3A , CD167 , DDR1 , NEP , NTRK4 , MCK10 , CAK , PTK3 , RTK6 , TRKE , DDR , HGK2 A |
|---|---|
| Target | DDR1 (phospho Tyr513) |
| Tissue Specificity | The antibody detects endogenous levels of DDR1 only when phosphorylated at tyrosine 513. |
Application
Western blot analysis of extracts from JK cells (Lane 2), using DDR1 (Phospho-Tyr513) Antibody. The lane on the left is treated with antigen-specific peptide.
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using DDR1 (Phospho-Tyr513) antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | WB: 1:500 - 1:1000. IHC: 1:50 - 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C/1 year |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
