Application
Immunofluorescence analysis of methanol-fixed HeLa, using DDX1 antibody at 1:200 dilution.Application
Sample (30 µg of whole cell lysate) A: A431 B: H1299 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
DDX1 antibody [N3C2], Internal detects DDX1 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. DDX1 antibody [N3C2], Internal dilution: 1:500.Application
DDX1 antibody [N3C2], Internal detects DDX1 protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. DDX1 antibody [N3C2], Internal dilution: 1:500.| Product Name | DDX1 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DDX1 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 1)DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DDX1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Chicken, Rhesus Monkey, BovineZebrafish (89%), Xenopus laevis (88%), Chicken (93%), Rhesus Monkey (98%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DBP-RB antibody UKVH5d antibody DDX1 antibody ATP-dependent RNA helicase DDX1 antibody DEAD box protein retinoblastoma antibody DEAD box protein 1 antibody DEAD box polypeptide 1 antibody DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 1 antibody DEAD |
|---|---|
| Molecular Weight(MW) | 82 kDa (note) |
| Tissue Specificity | Knockdown/Knockout validation was supported by references (PMID:26990997) |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using DDX1 antibody at 1:200 dilution.
Application
Sample (30 µg of whole cell lysate) A: A431 B: H1299 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
DDX1 antibody [N3C2], Internal detects DDX1 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. DDX1 antibody [N3C2], Internal dilution: 1:500.
Application
DDX1 antibody [N3C2], Internal detects DDX1 protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. DDX1 antibody [N3C2], Internal dilution: 1:500.| Positive Control | A431 , H1299 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
