Application
DDX18 antibody detects DDX18 protein by Western blot analysis. A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extract C. 30 µg NCI-H929 whole cell lysate/extract 7.5 % SDS-PAGE DDX18 antibody dilution: 1:1000Application
DDX18 antibody detects DDX18 protein at nucleolus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: DDX18 protein stained by DDX18 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | DDX18 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DDX18 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 18)DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, and it is activated by Myc protein. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DDX18. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevisMouse (97%), Rat (96%), Zebrafish (91%), Xenopus laevis (91%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DEAD (Asp-Glu-Ala-Asp) box polypeptide 18 MrDb |
|---|---|
| Target | DDX18 |
Application
DDX18 antibody detects DDX18 protein by Western blot analysis. A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extract C. 30 µg NCI-H929 whole cell lysate/extract 7.5 % SDS-PAGE DDX18 antibody dilution: 1:1000
Application
DDX18 antibody detects DDX18 protein at nucleolus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: DDX18 protein stained by DDX18 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | Jurkat , Raji , NCI-H929 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
