Application
Sample (30 ug of whole cell lysate) A:IMR32 7.5% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using DDX28 antibody at 1:500 dilution.| Product Name | DDX28 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DDX28 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 28)DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene is intronless. It encodes an RNA-dependent ATPase. The encoded protein is localized in the mitochondria and the nucleus, and can be transported between the mitochondria and the nucleus. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DDX28. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | BovineBovine (83%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ11282 antibody, MDDX28 antibody, DDX28 antibody, mitochondrial DEAD box protein 28 antibody, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 28 antibody, probable ATP-dependent RNA helicase DDX28 antibody, mitochondrial DEAD-box polypeptide 28 antibody, DE |
|---|---|
| Molecular Weight(MW) | 60 kDa (note) (2) |
Application
Sample (30 ug of whole cell lysate) A:IMR32 7.5% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using DDX28 antibody at 1:500 dilution.| Positive Control | NT2D1 , IMR32 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
