Application
WB analysis of extracts from various cell lines using DDX3 antibody.Application
ICC/IF analysis of U20S cell using DDX3 antibody (red) and DAPI (blue).Application
ICC/IF analysis of HeLa cells using DDX3 antibody (green) and DAPI (blue).| Product Name | DDX3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DDX3DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which interacts specifically with hepatitis C virus core protein resulting a change in intracellular location. This gene has a homolog located in the nonrecombining region of the Y chromosome. The protein sequence is 91% identical between this gene and the Y-linked homolog. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jul 2010] |
| Immunogen | Recombinant protein of human DDX3X |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPRIPWB |
| WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200. IP: 1:20 - 1:50. RIP: 1:20 - 1:50 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked , DBX , HLP2 , DDX14 , DDX3X |
|---|---|
| Target | DDX3 |
| Molecular Weight(MW) | 73 kDa (note) |
Application
WB analysis of extracts from various cell lines using DDX3 antibody.
Application
ICC/IF analysis of U20S cell using DDX3 antibody (red) and DAPI (blue).
Application
ICC/IF analysis of HeLa cells using DDX3 antibody (green) and DAPI (blue).| Application Notes | WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200. IP: 1:20 - 1:50. RIP: 1:20 - 1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
