Application
DDX4 antibody [C1C3] detects DDX4 protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. DDX4 antibody [C1C3] diluted at 1:500.Application
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using DDX4 antibody at 1:250 dilution.Application
Sample (30 µg of whole cell lysate) A: U87-MG 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | DDX4 antibody [C1C3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DDX4 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 4)DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human DDX4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWBIHC-W |
| WB:1:200~1:500 *Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseFish |
| Predicted Reactivity | Rat, Pig, Rhesus Monkey, Chimpanzee, BovineRat (90%), Pig (98%), Rhesus Monkey (99%), Chimpanzee (100%), Bovine (94%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC111074 antibody, VASA antibody, DDX4 antibody, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4 antibody, DEAD box protein 4 antibody, vasa homolog antibody, probable ATP-dependent RNA helicase DDX4 antibody, DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 antib |
|---|---|
| Molecular Weight(MW) | 79 kDa (note) (5) |
Application
DDX4 antibody [C1C3] detects DDX4 protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. DDX4 antibody [C1C3] diluted at 1:500.
Application
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using DDX4 antibody at 1:250 dilution.
Application
Sample (30 µg of whole cell lysate) A: U87-MG 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | NT2D1 , IMR32 , U87-MG , MCF-7 |
|---|---|
| Application Notes | WB:1:200~1:500 *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
