Application
Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using DDX47 antibody at 1:250 dilution.| Product Name | DDX47 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DDX47 (probable ATP-dependent RNA helicase DDX47)This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene can shuttle between the nucleus and the cytoplasm, and has an RNA-independent ATPase activity. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DDX47. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Chicken, Chimpanzee, BovineMouse (96%), Rat (96%), Xenopus laevis (86%), Chicken (86%), Chimpanzee (100%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp564O176 antibody, E4-DBP antibody, FLJ30012 antibody, HQ0256 antibody, MSTP162 antibody, RRP3 antibody, DDX47 antibody, DEAD box protein 47 antibody, DEAD box polypeptide 47 antibody, E4-DEAD box protein antibody, probable ATP-dependent RNA helicase D |
|---|---|
| Molecular Weight(MW) | 51 kDa (note) (2) |
| Tissue Specificity | Knockdown/Knockout validation was supported by references (PMID:26991014) |
| Cellular Localization | Nucleus , nucleolus |
Application
Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using DDX47 antibody at 1:250 dilution.| Positive Control | IMR32 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
